Interstate 65 (I-65) is the recurring character in Intertropolis & Routeville. She is a female Interstate Highway shield who is a musician, loves all kinds of music, and is a huge fan of a musical band called the New England Interstates (I-89, I-91, and I-93). She is one of the 23 members of the Main Interstate Highway signs living in the Main Highway Lounge of Intertropolis.